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KMID : 0385219990090010010
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Korean Journal of Gerontology
1999 Volume.9 No. 1 p.10 ~ p.17
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Reactive Oxygen Scavenging Activity of Green Tea Polyphenols
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Kye In-Sook
Chun Young-Soo
Noh Jae-Kyung
Kim You-Jung
Shim Kyung-Hee
Kim Jong
Yokozawa Takako
Chung Hae-Young
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Abstract
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Reactive oxygen species(ROS), such as O2.-, H2O2, and .OH, mediate reactions which can result in the damage of critical biological molecules including DNA and cellular protein. In the previous studies, green tea polyphenols were found to contain biological and pharmacological activities that may contribute to chemopreventive effects. However, almost no information was known on effects of green tea components against each specific ROS such as .OH, .O2.- and H2O2. The purpose of this present study was to investigate the ROS scavenging effects of green tea polyphenols against each species of ROS using DCFDA assay. Green tea polyphenols, (-)-epicatechin 3-O-gallate (ECG), (-)-gallocatechin 3-O-gallate (GCG), (-)-epigallocatechin 3-O-gallate (EGCG), (-)-epigallocatechin (EGC), (-)-epicatechin (EC) and (+)-catechin (C) significantly showed ROS scavenging activity in liver and kidney homogenate. Especially, ECG, GCG and EGCG showed the potent ROS scavenging activity, which indicates that the galloyl group may be active chemical moiety that is responsible for this scavenging activity. We also examined the effects of ECG, GCG and EGCG on ROS to elucidate the scavenging activity against .O2.-, .OH and/or H2O2. As the results, ECG, GCG, and EGCG remarkably scavenged the ROS. These results suggest that green tea polyphenols, ECG, GCG, and EGCG having multiple galloyl groups and flavan-3-ol, show potent antioxidative action through ROS scavenging activity.
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KEYWORD
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Green Tea Polyphenol, Reactive Oxygen Species(ROS), Scavenging Activity
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